gram stain Morphology B Cereus purple/blue rod bacillus B megaterium purple/blue rod bacillus arrangement pigment colony morphology growth in slant growth in broth sulfur (SIM) indole (SIM) motility (SIM) catalase oxidase Methyl Red (MR) vogues proskeur (VP) citrate Urease Gelatinase phenyl red dextrose phenyl red lactose phenyl red sucrose gas fluid thiolglycollate Phenylalanine starch agar plate Casein(skim milk) Lipase (TBA plate) CHAINS, STREPTO CHAINS, STREPTO flat undulate translucent FLAT, IRREGULAR flat undulate translucent FLAT, IRREGULAR SEDIMENT SEDIMENT ± FA OA ± B subtilis purple/blue rod bacillus E faecalis purple/blue spherical coccus L lactis purple/blue rod bacillus Gram Positive Chart M luteus purple/blue spherical coccus CHAINS, STREPTO ROUND, SMOOTH ROUND, SMOOTH SEDIMENT ± OA ± clusters, staphylo CONVEX MUCOID CONVEX MUCOID UFT ± ± ± FA ± CHAINS, STREPTO MUCOID, LOBATE MUCOID, LOBATE UFT ± FA ± ± FA OA ± UFT TETRADS Yellow ROUND, ENTIRE,SHINY, CONVEX ROUND, ENTIRE,SHINY, CONVEX SEDIMENT OA facultative anaerobe obligate aerobe 90% or greater positive 90% or greater negative 50% POSITIVE 50% NEGATIVE uniform fine turbidity M roseus purple/blue spherical coccus M phlei purple/blue spherical coccus S ureae purple/blue spherical coccus S aureus purple/blue spherical coccus S epiderm purple/blue spherical coccus S pyogenes purple/blue spherical coccus S salivarius purple/blue spherical coccus gram stain Morphology TETRADS red SMOOTH, CONVEX, ENTIRE SMOOTH, CONVEX, ENTIRE PELLICLE ± ± ± ± OA ± ± ± TETRADS SMOOTH, CONVEX, ENTIRE SMOOTH, CONVEX, ENTIRE PELLICLE ± ± OA – TETRADS, sarcinae RAISED, CIRCULAR, ENTIRE RAISED, CIRCULAR, ENTIRE UFT OA – clusters, staphylo RAISED, CIRCULAR, ENTIRE RAISED, CIRCULAR, ENTIRE UFT ± FA ± – clusters, staphylo RAISED, CIRCULAR, ENTIRE RAISED, CIRCULAR, ENTIRE SEDIMENT ± ± ± FA ± – CHAINS, STREPTO RAISED, CIRCULAR, ENTIRE RAISED, CIRCULAR, ENTIRE SEDIMENT FA ± – CHAINS, STREPTO RAISED, CIRCULAR, ENTIRE RAISED, CIRCULAR, ENTIRE UFT ± FA ± ± arrangement pigment colony morphology growth in slant growth in broth sulfur (SIM) indole (SIM) motility (SIM) catalase oxidase Methyl Red (MR) vogues proskeur (VP) citrate Urease Gelatinase phenyl red dextrose phenyl red lactose phenyl red sucrose gas fluid thiolglycollate Phenylalanine starch agar plate Casein(skim milk) Lipase (TBA plate)